Method of controlling algae with trichloroacrylonitrile

ABSTRACT

THIS INVENTION RELATES TO A METHOD OF CONTROLLING BACTERIA AND ALGAE BY APPLYING AN EFFECTIVE AMOUNT OF TRICHLOROACRYLONITRILE TO THE HABITAT THEREOF.

United States Patent 3,764,291 lVIETHOD 0F CONTROLLING ALGAE WITH TRICHLOROACRYLONITRILE Don R. Baker, Orinda, Califi, assignor to Staufier Chemical Company, New York, N.Y.

No Drawing. Original application May 26, 1971, Ser. No. 147,195, now Patent No. 3,711,603. Divided and this application July 17, 1972, Ser. No. 272,278

Int. Cl. A01n 9/20 US. Cl. 71-67 1 Claim ABSTRACT OF THE DISCLOSURE This invention relates to a method of controlling bacteria and algae by applying an eifective amount of trichloroacrylonitrile to the habitat thereof.

DESCRIPTION OF THE INVENTION This is a division of application Ser. No. 147,195, filed May 26, 1971, now Pat. No. 3,711,603.

This invention relates to the novel use of trichloroacrylonitrile. More particularly, the present invention involves the novel use of trichloroacrylonitrile as a bactericide and algaecide.

As a composition of matter, trichloroacrylonitrile is known and described in prior art and its method of preparation is likewise adequately described. Moreover, its use as a fungicide and nematocide is described in US. Pat. No. 3,145,138. However, there is no disclosure of the novel use of the subject compound as described here- The following tests clearly demonstrate the activity of trichloroacrylonitrile.

TEST 1 Algaecide and bactericide test This test measures the bactericidal and algicidal properties of a compound when a contact with a growing bacterium or algae in an artificial medium. The test is conducted by adding 20 ml. portions of a suitable warm steril agar solution into 20 x 100 mm. petri dishes. Next, the test compound (0.5% in acetone) is added to the petri dishes at levels of 1, 5, 10 and 50 ,ug./l11l. and mixed with the warm mobile agar solution. The treated agar mixture is then allowed to cool to room temperature and solidify. Cells of the particular organism are then streaked on the surface of the solidified agar and are then incubated for such lengths of time that the untreated samples containing no toxicant show luxurious growth typical of the ice Bacteria: for control (,ug./ml.) Pseudomonas aerugz'nosa (10) Bacillus cereus Algae:

Scenedesmus obliquus (50) Chlorella pyrinoz'dosa 50 indicates partial control at this concentration. Incubated at 37 C. Incubated at 30 C.

TEST 2 Sulfate reducing bactericidal test This test measures the bactericidal eifectiveness of a compound against sulfate reducing bacteria when grown in an artificial medium. The test is conducted using steril vials containing 9.0 ml. sulfate API broth with tryptone under anaerobic conditions. To these vials are added the test compound (05% in acetone) at levels to give 1, 5, 10 and 50 g/ml. final concentration. Next, to the vials are added 0.4 ml. steril distilled water and 0.5 ml. of the growing sulfate reducing bacterium innoculant. The vials are incubated at room temperature for one week and are then examined for growth of the organism. When Desulfovibrio desulfuricans is used as the organism in this test, trichloroacrylonitrile gave control at a level of 50 ,ug./ml.

What is claimed is:

1. The method of inhibiting the growth of algae comprising applying an effective amount of trichloroacrylonitrile to the habitat thereof.

References Cited UNITED STATES PATENTS 3,250,667 5/1966 Legator 7167 X 2,617,721 11/1952 Schlesinger et al 71-105 OTHER REFERENCES Lovless et al.: Chem. Abst., vol. 49, 4079i.

LEWIS GOTTS, Primary Examiner C. L. MILLS, Assistant Examiner 

